Uses And Options For The Synaptophysin Antibody

by | Dec 7, 2016 | Science and Technology

The Synaptophysin Antibody can recognize proteins with a molecular weight of 38kDa. It can label normal neuroendocrine cells within the human adrenal medulla, skin, carotid body, pituitary gland, lung, thyroid, pancreas, Paneth’s cells, gastrointestinal mucosa and more. Likewise, it can label the neurons in the spinal cord, retina, and brain. It is intended for research only and isn’t designed for diagnostic procedures. Likewise, it comes in sizes of 7.0 ml, 0.5 ml, 1.0 ml, and 0.1 ml.

How To Use It In Applications

The Synaptophysin Antibody can be utilized for Immunohistochemistry (IHC). To do so, you will need to prepare the specimens (both paraffin-embedded and formalin-fixed tissues can be used). You will need to deparaffinize the slides using graded alcohols and xylene or its alternatives, as well. Likewise, you will need to dilute it using a ratio of 1:300, though the dilution amount is an estimate.

To retrieve the antigens, you’ll need to boil your tissue sections for ten minutes and then cool for 20 minutes at room temperature. Likewise, incubation is at room temperature for 10 minutes.

The positive control includes the pheochromocytoma and the pancreas.

Other Information

The product should be stored at two to eight degrees Celsius and may require other storage needs. The reagent will remain stable at this temperature until the date indicated. Once the expiration date has passed, it should not be used and should be disposed of properly according to state laws.

There are no symptoms of instability, so it is best to use both negative and positive controls at the same time when dealing with unknown specimens. Likewise, it should not be contaminated with microbial reagents.

The Synaptophysin Antibody can help you learn more about the pancreas and other areas of the body. Visit Spring Bioscience now to find out more.

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