FOXP3 is a 431 amino acid protein and a member of the forkhead/winged-helix transcriptional regulator family. It is necessary for normal immune homeostasis and is constitutively and stably expressed at high levels in CD35+CD4 positive T-cells. It is also found in low levels in CD4 positive/CD25 negative T-cells and isn’t there in CD4 negative/CD8 positive cells. It is a more specific marker of T-cells than others and may be a master regulatory gene.
The FOXP3 antibody has no clone available, and the immunogen is the synthetic peptide from the c-terminus of the same protein in humans. The isotype is the Rabbit IgG, and it has an undetermined epitope. Its molecular weight is 50kDa.
This antibody can be used in Immunohistochemistry applications. To prepare your specimens, make sure you’re using paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides are also required and can be achieved using xylene, one of its alternatives, or graded alcohols.
You’ll find for purchase a variety of sizes, including a pre-diluted version. You can use the pre-diluted formula if it requires a dilution ratio of 1:200. If you will need a different ratio or want to do it yourself to be sure, you can also find a concentrated version.
To retrieve the antigen, you should boil the tissue sections for 10 minutes in a 1mM EDTA with a pH of 8.0. Then, you should wait 20 minutes for the concoction to come to room temperature. An incubation period is also necessary for 30 minutes while at room temperature.
The positive control for this test is the tonsil with cellular localization occurring in the nucleus.
The FOXP3 antibody can be used for a variety of research purposes. Visit Spring Bioscience today to learn more and purchase some for your lab.
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